Fig. 2.

Threshold of activation of cold-sensitive neurons from Na_V1.8^Cre/+ and Na_V1.8 ^Cre/Cre null DRG, in vivo. (A) Representative changes in GCaMP3 fluorescence from threshold-specific cold-responsive DRG neurons in response to a series of ∼−Δ5 °C changes in stimulus temperature, applied at the plantar surface. (B) Normalized summary of the threshold of activation of cold-sensitive DRG neurons from Na_V1.8^Cre/+ (n = 4) and Na_V1.8^Cre/Cre null (n = 5) mice. (C and D) Summary of the number of cold-responsive neurons with or without tomato fluorescence, from Na_V1.8^Cre/+ (C) and Na_V1.8^Cre/Cre null (D) DRG neurons.