Fig. 2.

Knockdown of β3GalT5 caused cell apoptosis and inhibition of cell metastasis. (A) The migration ability of MDA-MB-231 cells measured by wound-healing assay. Cells infected with sh β3GalT5 were treated with or without Z-DEVD-FMK, a caspase-3 inhibitor, and incubated for 18 h. Representative data among triplicated experiments are shown. (Scale bars, 100 μm.) (B and C) The invasion (B) and adhesion (C) abilities of MDA-MB-231 cells with or without β3GalT5 knockdown. The percentage of invaded cells were measured with calcium AM. (D) Western blot analysis of caspase-3 activation and cleavage of FAK in MDA-MB-231 cells with β3GalT5 knockdown. (E) Expression of caspase-3 and FAK in MDA-MB-231 cells in the presence or absence of caspase-3 inhibitor. Bars represent the mean ± SD values from three experiments. The P value was obtained by t test. *P < 0.05; **P < 0.01.