PILS6 regulates root growth under HT. (A and B) PILS6 affects root growth under HT. Scanned images, quantification of the root segment grown after 3-d exposure to 21 °C (control) or 29 °C (HT), and calculated ratio showing that pils6-1 and PILS6OE affect root growth under HT compared with the wild type (A). Confocal images and (relative) quantification of wild type, pils6-1, and PILS6OE root meristems exposed for 3 d to 21 °C or 29 °C (B). Root meristem is depicted as the distance between the quiescent center and the uppermost first cortical cell that is twice as long as it is wide. [Scale bars, 500 μm (A) and 100 μm (B).] n = 32–34 roots (A) and 20 root meristems (B). *P = 0.01–0.05, **P = 0.001–0.01, ***P < 0.001, one-way ANOVA.