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. 2019 Feb 13;116(9):3899–3908. doi: 10.1073/pnas.1816652116

Fig. 6.

Fig. 6.

Overexpression of DMS3 mimics impaired RdDM activity in dms3 and apc/c. (A) qRT-PCR to determine transcript levels of the RdDM loci AtSN1, AtGP1, soloLTR, SDC, At1TE89775, At4TE34015, At3TE13000, and At3TE47905 in Col-0, apc8-1, and DMS3-OE plants. Total RNA from inflorescences was analyzed. The UBQ5 gene was amplified as an internal control. Data are presented as mean ± SE (n = 3). (B) CHH methylation of AtSN1, SDC, soloLTR, and At3TE47905 was determined by bisulfite sequencing in Col-0, apc8-1, and DMS3-OE plants. (C) Western blotting analysis detected the endogenous DMS3 levels of root tips at indicated time points after HU treatments. DMS3 levels using anti-DMS3 antibody (Top), the CYCB1;1 level using anti-GFP antibody (a positive control regulated by APC/C) (Middle), and the loading control using anti-Hsc70 antibody (Bottom) are indicated. R1, biological replicate 1; R2, biological replicate 2.