Fig. 1.

(A) Illustration of different regions defined in the image analysis. (B) Illustration of the selected regions of tumor margin and tumor core. For the tumor margin, parts of the margins were excluded whenever the juxtatumoral tissue did not exist. For the tumor core, whenever possible three regions ($1.95\hspace{0.17em}\mathrm{m}\mathrm{m}\times 1.95$ mm) were selected for each tumor. (C) Illustration of the ${\mathrm{C}\mathrm{D}\mathrm{8}}^{+}$–T cell profile calculation for two levels: margin-boundary level and tumor cell cluster level (Materials and Methods). (D) Based on the spatial profile of ${\mathrm{C}\mathrm{D}\mathrm{8}}^{+}$ T cells at the tumor margin and the tumor core, 28 patients were grouped into two groups (margin-boundary level) and three groups (tumor cell cluster level), respectively.