Fig. 5.

Disease space maps of SC- and WD-fed mice during LPS-driven sepsis. (A) Two independent disease markers, temperature (Temp) and neutrophil (Neuts) number in the blood, were plotted against each other over multiple time points (0–20 h p.t.) to create a disease space map for SC-fed (black) and WD-fed (green) mice after a single i.p. injection of 6 mg/kg of LPS. Green shading indicates the portion of the disease map occupied only by WD-fed mice. (B–F) Temperature (y axis) and neutrophil number in the blood (x axis) after i.p. injection of LPS were plotted against each other for each time p.t. Ellipses indicate 70% confidence intervals for the mean of each experimental group, SC fed (gray shading) and WD fed (green shading). A total of four to eight mice per group was used, and each independent experiment was repeated three times. For B–F, multivariate analysis of variance was used to show significant differences in both the temperature and neutrophil number in the blood between the two experimental groups. For panels B–F 0 h, not significant; 5 h, **P < 0.001; 10 h, **P < 0.002; 20 h, *P < 0.047; and 25 h, **P < 0.002.