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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: J Mol Biol. 2018 Dec 29;431(4):842–856. doi: 10.1016/j.jmb.2018.12.014

Figure 2. Biochemical characterization of PR8 D190 GCN4IL-sfGFP.

Figure 2.

(A) Biological activity as measured by hemagglutination using human red blood cells. (B) Purified HA-sfGFP fusion proteins analyzed with conformational dependent stalk antibodies and polyclonal mouse serum. (C) Melting curves of recombinant HA proteins with or without a sfGFP fusion by Differential Scanning Calorimetry. Individual melting curves are shown with the raw data depicted in solid lines, while the fitted curves, from which the Tm values were derived, are depicted with a dotted line.