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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: J Mol Biol. 2018 Dec 29;431(4):842–856. doi: 10.1016/j.jmb.2018.12.014

Figure 6. Tissue stainings of PR8 D190 and PR8 D190E .

Figure 6.

(A) Staining of chicken trachea with PR8 D190 and pig primary bronchus with PR8 D190E (B) Staining of tissues with PR8 D190 and PR8 D190E precomplexed with α-strep-tag mouse antibody and goat-α-mouse-HRP in a 4:2:1 molar ratio (C) Staining of tissue with PR8 D190 and PR8 D190E precomplexed with α-strep-tag-488 conjugated mouse antibody and goat-α-mouse-488 conjugated antibody in a 4:2:1 molar ratio (D) Left: lectin histochemistry using the PR8 proteins precomplexed with HRP labelled antibodies using AEC as a readout. Middle, neuraminidase treatment of the tissues and stained with PR8 D190 for pig bronchus (left) and PR8 D190E on chicken trachea (right) to deterimine sialic acid dependency. Right, reciprocal stainings using PR8 D190 on chicken trachea and PR8 D190E on pig bronchus to confirm sialic acid linkage specificity of both proteins, respectively.