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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Cancer Immunol Res. 2019 Jan 4;7(3):414–427. doi: 10.1158/2326-6066.CIR-18-0126

Figure 2. SUV39H1 expression is elevated in both colon carcinoma cells and tumor-infiltrating CTLs.

Figure 2.

A. Data sets of SUV39H1 mRNA in human colon carcinoma (n=380) and normal colon tissues (n=51) extracted from TCGA database and plotted as a heatmap shown in the left panel. The SUV39H1 mRNA expression range is represented by red and blue color gradient as indicated at the left bottom panel. The relative mRNA expression is represented as Log2 (normalized counts+1). The SUV39H1 mRNA levels was compared between colon carcinoma and normal colon tissues and presented in the right panel. Two-tail t tests were used to determine differences in SUV39H1 mRNA expression between tumor tissues and normal colon tissues with p<0.05 as being statistically significant. Bar: Mean. B. Tumors were excised from two MC38 tumor-bearing mice and digested in a collagenase solution. Portion of the tumor cells were incubated with anti-CD45–conjugated magnetic beads to deplete leukocytes from tumor cells. Another portion of the tumor cells was incubated with anti-CD8–conjugated magnetic beads to isolated CD8+ tumor-infiltrating CTLs. RNA was prepared and analyzed by qPCR using SUV39H1-specific primers. The two open bars indicate SUV39H1 mRNA in CD8+ tumor-infiltrating CTLs from two tumor-bearing mice. The two grey filled bars represent SUV39H1 mRNA in CD45-depleted tumor cells from two tumor-bearing mice. Bottom panel: agarose gel showing the PCR-amplified Suv39h1 DNA fragment. Rpl13a was used as normalization control.