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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 24;564-565:96–101. doi: 10.1016/j.ab.2018.10.022

Figure 2.

Figure 2.

Apoptotic response in C57BL/6J MLFs and HT1080 fibrosarcoma cells, as quantified by microplate reader. Cells were seeded at 35,000 cells/mL in 96-well plates and treated with doses of staurosporine (STS) ranging from 0.00 μM to 1.50 μM, as well as 1X IncuCyte® Caspase-3/7 Green Reagent. Using a microplate reader, cellular apoptosis was assessed at 6 timepoints over 24 hr by quantifying fluorescence signal from activated IncuCyte® Caspase-3/7 Green Reagent (A and C). Rates of apoptosis in response to each dose of STS were determined by linear regression and plotted against corresponding dose of STS (B and D). Goodness of fit for dose-response curves in B and D (r2 = 0.9924 and 0.9999, respectively) were determined by sigmoidal least squares nonlinear regression. Values for each timepoint in A and C represent mean and SEM of 3 replicates and are normalized to control wells (0.00 μM STS) to control for baseline apoptosis over 24 hr.