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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 24;564-565:96–101. doi: 10.1016/j.ab.2018.10.022

Figure 3.

Figure 3.

Fluorescent microscopy of cells treated with IncuCyte® Caspase-3/7 Green Reagent. C57BL/6J MLFs were seeded into microplates at 35,000 cells/mL and treated with either vehicle only (A), IncuCyte® Caspase-3/7 Green Reagent only (B), or 1 μM STS plus 1X IncuCyte® Caspase-3/7 Green Reagent (C). Cells were allowed to incubate at 37°C for 12 hr, then imaged by fluorescent microscopy. Brightfield images show morphological changes in apoptotic cells treated with STS (C, as compared to A and B), and images taken in FITC channel show fluorescing IncuCyte® Caspase-3/7 Green Reagent, which localizes to nuclei and is indicative of caspase cleavage. Original magnification 40x; scale bar indicates 100 μm.