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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Anal Biochem. 2018 Oct 24;564-565:96–101. doi: 10.1016/j.ab.2018.10.022

Figure 4.

Figure 4.

Hoechst 33342 live cell nuclear staining and fluorescence quantification. C57BL/6J MLFs were serially diluted and plated at various known seeding densities ranging from 1,000 cells/mL to 40,000 cells/mL (n = 8 wells per seeding density) and stained with Hoechst 33342. Fluorescent signal was quantified by microplate reader and shown to be highly correlated to known seeding density (A; linear regression r2 = 0.8812). In B, cells seeded at 40,000 cells/mL and stained with Hoechst 33342 and imaged by fluorescence microscopy. Original magnification 40x; scale bar indicates 100 μm.