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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Methods. 2018 Nov 22;156:85–90. doi: 10.1016/j.ymeth.2018.11.007

Fig. 2.

Fig. 2.

Use of a 3’ adaptor with an inline barcode prevents mispriming. Mispriming may occur on fragments containing an internal sequence complementary to the 3’ terminus of the RT primer sequence (left). However, only cDNA products produced from correctly primed products will contain the in-line barcode (5’ CGTGAT 3’), which is underlined in the figure. Reads without the in-line barcode are filtered out, thus preventing mispriming.