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Interpreted MS/MS spectrum obtained on the Synapt G2-S for (A) [m7G]UC[m5C]UG and (B) A[Cm]U[Gm]AA[yW]AU[m5C]UG digestion products from S. cerevisiae tRNAPhe. Due to the significant neutral base losses of [m7G] of [yW] during CID, [M-BH] and product ions containing these modified bases are not assigned.
