Table 1.
Strains and plasmids used in this work.
| Strains and plasmids | Description | Source |
|---|---|---|
| Strains | ||
| GSL | C. glutamicum ATCC13032 with the following modifications: Δpck, ΔsugR, ΔldhA, pycP458S, homV 59A, two copies of lysCT311I, two copies of asd, two copies of dapA, two copies of dapB, two copies of ddh, two copies of lysA, two copies of lysE, in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071) | Pérez-García et al., 2016 |
| GSLΔyggB | In-frame deletion of yggB (cg1434) in GSL | This work |
| GSLΔproP | In-frame deletion of proP (cg3395) in GSL | This work |
| JJ001 | C. glutamicum ATCC13032 with the following modifications:ΔargF, ΔargR (auxotrophic for L-arginine); carrying the vector pVWEx1 | Jensen and Wendisch, 2013 |
| JJ004 | JJ001 strain carrying the vector pVWEx1-ocdPp(TAA) | Jensen and Wendisch, 2013 |
| E. coli DH5α | F-thi-1 endA1 hsdr17 (r-, m-) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 relA1 | Hanahan, 1983 |
| E. coli S17-1 | recA, thi, pro, hsd R-M+ (RP4: 2-Tc:Mu-:Km, integrated into the chromosome) | Simon et al., 1983 |
| Plasmids | ||
| pVWEx1 | KmR, C. glutamicum/E. coli shuttle vector (Ptac, lacI, pHM1519 oriVCg) | Peters-Wendisch et al., 2001 |
| pEKEx3 | SpecR, C. glutamicum/E. coli shuttle vector (Ptac, lacI, pBL1 oriVCg) | Stansen et al., 2005 |
| pVWEx1-lysDH-proC | KmR, pVWEx1 overexpressing lysDH from S. pomeroyi DSS-3 and proC from C. glutamicum ATCC 13032 | Pérez-García et al., 2016 |
| pEKEx3-yggB | SpeR, pEKEx3 overexpressing yggB from C. glutamicum ATCC 13032 | This work |
| pEKEx3-proP | SpeR, pEKEx3 overexpressing proP from C. glutamicum ATCC 13032 | This work |
| pK19mobsacB | KmR; E. coli/C. glutamicum shuttle vector for construction of insertion and deletion mutants in C. glutamicum (pK18 oriVEc sacB lacZα) | Schaffer et al., 2001 |
| pK19mobsacB-ΔyggB | pK19mobsacB with a yggB (cg1434) deletion construct | Lubitz and Wendisch, 2016 |
| pK19mobsacB-ΔproP | pK19mobsacB with a proP (cg3395) deletion construct | This work |