Figure 6.

Rescue of morphant MCC ciliation is lost with mutant PIBF1 alleles. (A–E) MCC ciliation in WT embryos was lost in pibf1 TBMO-injected specimens. (E–G) Rescue of ciliation upon co-injection of TBMO and EGFP-PIBF1. (E,H,I) Attenuated rescue in embryos co-injected with missense PIBF1 allele. (E,J,K) Defective rescue upon co-injection of the nonsense PIBF1 allele. (E) Quantification of results. (L,M) Kymographs representing ciliary beating in WT and TBMO-injected stage 30 larval skin MCCs. ***Very highly significant, p < 0.01.