Figure 1.

Impaired effector responses and activating Fc receptor-expression in FcRγ-deficient neutrophils. Wild type (WT) and FcRγ-deficient (FcRγ KO) neutrophils were plated on immobilized immune complexes and their superoxide release (A), gelatinase degranulation (B), and spreading (C) was followed. The ability of FcRγ KO cells to produce superoxide anions was tested by PMA stimulation (at a 100 nM concentration) (D). Neutrophil cell surface FcγRIV expression was detected by flow cytometry (E). The gelatinase zymogram, the photos and the flow cytometric curves are representative of 3–7 independent experiments. Kinetic curves in (A,D) show mean and SEM of 3 independent experiments. Control data points were subtracted. See the text for actual P values. IC, Immune complex; PMA, Phorbol myristate acetate.