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. 2019 Feb 25;10:252. doi: 10.3389/fimmu.2019.00252

Figure 5.

Figure 5

Dose-dependent effect of the wild type transgene on the severity of autoimmune arthritis in contrast to complete protection of the ITAM tyrosine mutant animals. FcRγ expression was tested by immunoblotting on whole cell lysates of neutrophils (A), while cell surface expression of the FcγRIV was followed by flow cytometry (B). (C–E) Wild type (WT), FcRγ-deficient (FcRγ KO), double wild type FcRγ transgenic (FcRγ KO + 2x WT FcRγ Tg) or double ITAM tyrosine mutant FcRγ transgenic (FcRγ KO + 2x YF FcRγ Tg) bone marrow chimeras were injected with B × N (Control) or K/B × N (Arthritic) serum intraperitonally on Day 0. Arthritis development was followed by clinical scoring of the fore limbs (C) and the hind limbs (D) and ankle thickness measurement (E). The blot and the flow cytometric curves are representative of 4 independent experiments. Quantitative data show mean and SEM from 4 to 5 control and 5 to 6 arthritic serum-treated individual mice per group from 2 independent experiments (C–E). See the text for actual P values.