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. 2019 Feb 25;10:162. doi: 10.3389/fpls.2019.00162

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Copyright © 2019 Mücke, Reschke, Erkes, Schwietzer, Becker, Streubel, Morgan, Wilson, Grau and Boch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow of TALE target gene identification. (A) Identification of candidate target genes. The top panel describes the steps for in silico predictions and the bottom panel shows the in vivo transcriptome analysis. (B) Venn diagram of intersections between predicted target genes and genes induced upon Xoo infection. (C,D) Strategy to evaluate individual candidate target genes. (C) Steps to identify the influence of single TALEs on target gene expression. (D) Steps to assess interactions between individual TALEs and their respective target promoters.