Table 2.
Mean fluorescent intensity and corresponding calculated DNA content per cell (DNAC) (Mb) of Sporothrix genus strains estimated by flow cytometry (FCM) of SYBR Green I stained cells.
| Isolate identification | (MFISGI ± SD a ) × 103 | CV b | DNAC ± SD (Mb) c | Genome size (Mb) d | Ploidy ratio e |
|---|---|---|---|---|---|
| S. brasiliensis | |||||
| ATCC MYA-4823 | 101.13 ± 0.58 | 4.42 | 29.89 ± 0.10 | 33.2 | 0.9 |
| IPEC 27454 | 89.26 ± 1.09 | 3.49 | 25.43 ± 0.11 | – | 0.8 |
| HUPE 114158 | 88.97 ± 0.13 | 4.00 | 25.32 ± 0.11 | – | 0.8 |
| IPEC 25374 | 100.27 ± 0.36 | 4.28 | 29.57 ± 0.10 | – | 0.9 |
| S. schenckii s. str. | |||||
| ATCC MYA-4821 | 85.80 ± 0.84 | 3.53 | 24.13 ± 0.12 | 32.4 | 0.7 |
| S. globosa | |||||
| CBS 120340 | N.D. | N.D. | N.D. | 33.4 | N.D. |
| 14,879/07 | 97.15 ± 0.33 | 5.65 | 28.40 ± 0.10 | – | 0.9 |
| S. pallida | |||||
| SPA8 | N.D. | N.D. | N.D. | 37.8 | N.D. |
| MUM 17.04 | 109.71 ± 3.28 | 6.40 | 33.12 ± 0.10 | N.D. | 0.9 |
a
Mean fluorescence intensity (MFISGI) of cells in G0/G1 phases of the cell cycle profile.
b
Coefficient of variation (CV) for the G0/G1 cell population.
c
DNA content per cell, in megabases, estimated by FCM.
d
Genome size, in megabases, reported in the literature (Teixeira et al., 2014; D’Alessandro et al., 2016; Huang et al., 2016).
e
Ratio of the genome size estimated by FCM and the reported value; N.D., not determined.