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. 2019 Mar;60(3):335–345. doi: 10.1165/rcmb.2018-0122OC

Figure 1.

Figure 1.

Myeloid-specific deletion of TNF receptor–associated factor family member–associated NF-κB activator–binding kinase 1 (TBK1) impairs IFN regulatory factor 3 (IRF3) phosphorylation in macrophages. (A) RT-qPCR of TBK1 mRNA from mouse embryonic fibroblasts, bone marrow macrophages (BMMs), and BAL macrophages. Error bars represent technical triplicates, and data are representative of two independent experiments. (B) Immunoblots of BMMs from wild-type (WT; LysM-Cre−, TBK1flox/flox) or TBK1-knockout (M-TBK1-KO; LysM-Cre+, TBK1flox/flox) mice stimulated in vitro with 100 ng/ml LPS or 25 μg/ml polyinosinic:polycytidylic acid [poly(I:C)] for the indicated times. (C) Immunoblots of alveolar macrophages from WT or M-TBK1-KO mice stimulated in vitro with the same concentrations of LPS or poly(I:C). (D) Immunoblots of alveolar macrophages from WT or multiple M-TBK1-KO mice. Immunoblots are representative of at least two independent experiments. AM = alveolar macrophage; H3 = histone H3; ISG15 = IFN-stimulated gene 15; US = unstimulated.