Figure 3.

Low S100A8 expression in ATII cells from patients with emphysema. (A and B) Western blot images of expression and densitometric quantification of S100A8 protein (A) and mRNA levels (B) in freshly isolated ATII cells from NS, SM, and patients with E. Quantification of S100A8 expression was normalized to β-actin and control nonsmokers (n = 4). (C) ATII cells obtained from nonsmokers were treated with 1%, 5%, or 10% cigarette smoke extract (CSE) for 24 hours to determine S100A8 expression by Western blotting and densitometric analysis (n = 3). *P < 0.05; **P < 0.001. Data are shown as means ± SD. ATII = alveolar type II.