Figure 6.

S100A8 knockdown sensitizes cells to apoptosis. (A) A549 cells were transfected with 15, 30, and 60 nM S100A8 siRNA or nontargeting (NT) siRNA for 48 hours. Cell lysates were subjected to Western blotting to determine S100A8 expression. Densitometric quantification of S100A8 expression was normalized to control (CTL). (B and C) A549 cells were transfected with 60 nM NT siRNA or S100A8 siRNA followed by exposure to 20% CSE for 24 hours. Representative images of flow-cytometric analysis using double staining with Annexin V and propidium iodide (PI) and quantification are shown (n = 3). *P < 0.05. Data are shown as means ± SD.