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. 2019 Feb 18;2019:7192798. doi: 10.1155/2019/7192798

Figure 2.

Figure 2

High glucose enhanced bupivacaine-induced MCU expression elevation. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) MCU mRNA was measured with qRT-PCR in cells treated with 1.0 mM bupivacaine for different times (3, 6, or 12 h). (b) MCU mRNA was measured with qRT-PCR in cells treated with 25 mM glucose for different times (1, 2, or 4 days). (c) MCU protein expression was measured with western blotting in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Values are the mean ± SD (n = 3); ∗P < 0.05, ∗∗P < 0.01.