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. 2019 Feb 18;2019:7192798. doi: 10.1155/2019/7192798

Figure 4.

Figure 4

Ru360 or the knockdown of MCU could attenuate bupivacaine-induced cell viability inhibition and oxidative damage in SH-SY5Y cell incubation with high glucose. Con: untreated cells; Vehicle: cells cultured with media containing DMSO; NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Cell viability was measured with MTT assay in cells cultured with 5, 10, or 20 μΜ Ru360 for 30 min and then treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (b) The 8-OHdG level was measured with ELISA in cells cultured with 5, 10, or 20 μΜ Ru360 for 30 min and then treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (c) MCU protein expression was measured with western blotting in cells treated with silencer negative control or MCU siRNA. (d) MCU protein expression was measured with western blotting in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (e) Cell viability inhibition was measured with MTT assay in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (f) The 8-OHdG level was measured with ELISA in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. Values are the mean ± SD (n = 3); ∗P < 0.05, ∗∗P < 0.01.