Figure 5.

Ru360 or the knockdown of MCU could attenuate bupivacaine-induced mCa²⁺ accumulation and mROS overproduction in SH-SY5Y cell incubation with high glucose. NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; Ru360: cells pretreated with 10 μΜ Ru360 for 30 min; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial Ca²⁺ was shown with the confocal scanning laser microscope. (b) Mitochondrial ROS was shown with the confocal scanning laser microscope. (c) Summarized data show mCa²⁺ measured with flow cytometry. (b) Summarized data show mROS measured with flow cytometry. Values are the mean ± SD (n = 3); ^∗P < 0.05, ^∗∗P < 0.01.