Skip to main content
. 2019 Feb 18;2019:3024970. doi: 10.1155/2019/3024970

Figure 2.

Figure 2

CNN3 is important for the invasiveness of MKN-28 gastric cancer cells. (a) MKN-28 cells were transfected with control (siCon) or two different CNN3-specific siRNAs (siCNN3-1 and siCNN3-2) using siLentFect Lipid Reagent. Western blot analysis was performed against indicated antibodies. (b) Control siRNA- and CNN3 siRNA-transfected MKN-28 cells were seeded onto Matrigel matrix-coated upper chambers. The invasion activity of each cell was represented as % of control cells. The data shown are expressed as the means ± SD of three individual experiments, each performed in triplicate. ∗P < 0.01 as determined by t-test. (c) Control siRNA- and CNN3 siRNA-transfected MKN-28 cells were analyzed in wound-healing assays by visualizing wound closure via phase-contrast microscopy. (d) Effect of CNN3 depletion on the proliferation of MKN-28 cells.