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EPR spectra (9.38 GHz CW) of P. aerophilum EtfAB and EtfABCX. The samples are as follows. AB AP, as-purified EtfAB; AB NADH, EtfAB treated with NADH; ABCX NADH, EtfABCX treated with NADH; AB DT, EtfAB treated with sodium dithionite; AB DT-NAD, EtfAB sequentially treated with sodium dithionite and NAD+; ABCX AP, EtfABCX as purified; ABCX NADH, EtfABCX treated with NADH. Top spectra (A–E) were recorded at 77 K, and bottom spectra (F and G) were recorded at 10 K.
