Polyamine supplementation counteracts protein hyperacetylation and decreased hatching rates in cts-1 RNAi embryos. A, left, acetylated proteins in mock RNAi embryos with vehicle (−) and cts-1 RNAi embryos with vehicle (−), putrescine (Put), or spermidine (Spd). Right, the levels of total acetylated proteins larger than 28 kDa in mock and cts-1 RNAi embryos supplemented with or without Put or Spd (data are mean ± S.E.; n = 3; ***, p < 0.001; *, p < 0.05). B, the hatching rate of mock RNAi embryos with vehicle (−) and cts-1 RNAi embryos with vehicle (−), Put, or Spd (data are mean ± S.E.; n = 3; *, p < 0.05; ***, p < 0.001).