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. 2019 Jan 3;294(9):3091–3099. doi: 10.1074/jbc.RA118.004726

Figure 6.

Figure 6.

sir-2.2 is necessary for recovery effect of spermidine on protein hyperacetylation and developmental arrest in cts-1 RNAi embryos. A, left, Western blotting of acetylated proteins of mock RNAi embryos and cts-1 RNAi embryos in WT and sir-2.2(tm2648). Right, the levels of total acetylated proteins larger than 28 kDa in mock and cts-1 RNAi embryos of N2 or sir-2.2(tm2648) (data are mean ± S.E.; n = 3; *, p < 0.05; N.S., not significant). B and C, left, acetylated proteins of mock RNAi embryos with vehicle (−) and cts-1 RNAi embryos with vehicle (−) or Spd in sir-2.2(tm2648) (B) and sir-2.3(ok444) (C) mutants. Right, the levels of total acetylated proteins larger than 28 kDa in mock and cts-1 RNAi embryos of sir-2.2(tm2648) (B) and sir-2.3(ok444) treated with or without Spd (data are mean ± S.E.; n = 3; ***, p < 0.001; **, p < 0.01; *, p < 0.05; N.S., not significant). D, the hatching rate of mock RNAi embryos with vehicle (−) and cts-1 RNAi embryos with vehicle (−) or Spd in N2, sir-2.1(ok434), sir-2.2(tm2648), and sir-2.3(ok444) mutants (data are mean ± S.E.; n = 3; **, p < 0.01; ***, p < 0.001; N.S., not significant).