Figure 3.

TGFβ promotes C/EBPβ expression. A, LX-2 cells were treated with 5 ng/ml TGFβ for 0, 1, 2, 4, or 24 h, harvested, and assessed by immunoblotting for expression of C/EBPβ isoforms (LAP1/2 and LIP). HSC70 served as a loading control. Quantification is shown below. B, shCollagen 1α1 or NT cells were treated with TGFβ for 0, 1, 2, 4, or 24 h, and cell lysates were analyzed by immunoblotting for collagen I or C/EBPβ isoforms. HSC70 served as a loading control. Quantification is shown below. C, LX-2 cells were treated with 5 ng/ml TGFβ for 4 h, fixed, permeabilized, and stained for C/EBPβ (green) and DAPI (blue). Representative images are shown. Statistics were performed using ANOVA followed by Tukey post hoc analysis (*, p < 0.05; **, p < 0.01). n ≥ 3 biological replicates. Error bars, S.E.