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Measurements of metal content in milk from TMEM165Δ/Δ and control mothers were taken. Concentrations of calcium (A), manganese (B), iron (C), and zinc (D) in skimmed milk samples from day 12 of lactation were measured by ICP-MS as described under “Experimental Procedures.” Milk from six control and six TMEM165 homozygous knockout animals were tested. *, p < 0.05; **, p < 0.01; ***, p < 0.001 using Student's t test.
