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Dose–response inhibition curves for pyrrophenone using an equal molar mixture of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphate (PAPA), PAPC, 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine (PAPE), PAPG, and PAPS as a substrate. (A) Activity of cPLA2 was measured by detecting 16:0 LPA, 16:0 LPC, 16:0 LPE, 16:0 LPG, and 16:0 LPS in a positive ion mode and (B) by detecting free AA in a negative ion mode.
