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. Author manuscript; available in PMC: 2019 Jul 15.
Published in final edited form as: Neuroscience. 2018 May 9;383:205–215. doi: 10.1016/j.neuroscience.2018.04.047

Figure 4. Effect of PLZ treatment on 5-HT+ immunoreactivity in the dorsal horn.

Figure 4

(A,B) Representative photomicrographs of 5-HT immunostaining in the lumbar dorsal horn in VEH (n=5) and PLZ (n=5) treated animals. Scale bar in E=100μm, applies in A-E. (A’,B’ inset) Higher magnification of superficial dorsal horn 5-HT staining. Scale bar=20μm. (C) Quantification (integrated density) of 5-HT+ immunoreactivity in the superficial (laminae I-II) of the bilaterally averaged dorsal horns (t-test, *p=0.020). (D,E) Representative photomicrographs of 5-HT immunostaining in the lumbar ventral horn (VH). (F) Quantification (integrated density) of 5-HT+ immunoreactivity in the (bilaterally averaged) ventral horn. (t-test, NS p=0.209)