Table I. MS/MS peptide identification for DMF-treated neurons and astrocytes. Raw data were searched with the Sequest node of Proteome Discover 1.4, using the uniprot_ref_mouse or uniprot_ref_rat databases. The variable modifications of methionine oxidation (M^OX), proline hydroxylation (P^ox), cysteine pyridylethylation (C^PE, 105.058), cysteine succination by fumarate (C^2SC, 116.011), cysteine modification by monomethyl fumarate (C^MMF, 130.026) or cysteine modification by dimethyl fumarate (C^DMF, 144.042) were considered.

Protein name [uniprot name]	Protein sequence % coverage	# Unique peptides	Modified sequence	Modification site	Modified peptide PSMs	XCorr	m/z	Charge	MH+[Da]
Primary Neurons
Collapsin Response Mediator Protein 2 [DPYL2]	74.48	21	GLYDGPVCDMFEVSVTPK	Cys 504	2	1.9	854.4096	2	1707.812059
Vimentin [VIME]	94.85	49	QVQSLTCDMFEVDALK	Cys 328	3	2.42	789.3899	2	1577.772631
Guanine nucleotide-binding protein G(o) subunit alpha [GNAO]	55.93	13	MOXVCDMFDVVSR	Cys 108	1	2.56	534.7373	2	1068.467333
Annexin A1 [ANXA1]	63.87	19	QACDMFYIEK	Cys 12	2	1.21	499.7269	2	998.4465808
Primary Astrocytes
Filamin alpha [C0JPT7]	59.49	106	YTP CMMFGAGSYTIMVLFADQATPTSPIR	Cys 841	1	1.85	969.4722	3	2906.402134
Myosin-9 [MYH9]	34.93	39	CMMFNGVLEGIR	Cys 694	10	1.21	545.7675	2	1090.524828
Heat shock protein HSP 90-alpha [HS90A]	69.3	21	VFIMOXDNCDMFEELIPERYLNFIR	Cys 375	3	2.24	840.0648	3	2518.180088
Heat shock protein HSP 90-beta [HS90B]	67.4	28	VFIMOXDSCDMFDELIPEYLNFIR	Cys 366	4	1.49	1239.089	2	2477.171678
Collapsin Response Mediator Protein 2 [DPYL2]	76.22	34	GLYDGPVCMMFEVSVTPK	Cys 504	2	3.08	847.402	2	1693.796801
			GLYDGPVCDMFEVSVTPK	Cys 504	3	1.83	854.4108	2	1707.814501
Vimentin [VIME]	91.85	50	QVQSLTCDMFEVDALK	Cys 328	3	2.25	789.3909	2	1577.774706
			QVQSLTCMMFEVDALK	Cys 328	3	1.65	782.3807	2	1563.754198
Glial fibrillary acidic protein [GFAP]	88.37	40	QLQALTCMMFDLESLR	Cys 292	1	1.37	810.4024	2	1619.797533
Annexin A1 [ANXA1]	69.94	22	GDRCDMFEDMOXSVNQDLADTDAR	Cys 189	1	2.04	757.6424	3	2270.912815
			QACDMFYIEK	Cys 12	1	1.06	499.7257	2	998.4441394
Cofilin-1 [COF1]	93.37	10	HELQANCMMFYEEVKDR	Cys 139	1	4.86	621.9409	3	1863.808201
Peptidyl-prolyl cis-trans isomerase A [PPIA]	73.17	9	VCDMFFELFADK	Cys 21	1	1.95	608.2839	2	1215.560594
Fatty acid-binding protein, brain [FABP7]	78.03	8	TQCDMFTFK	Cys 56	3	2	436.1958	2	871.3869494
N1E-115 Neurons
ATP-citrate synthase [ACLY]	51.77	39	YICMMFTTSAIQNR	Cys 20	2	1.57	700.3239	2	1399.640551
Ubiquitin carboxyl-terminal hydrolase isozyme L1 [UCHL1]	58.3	13	NEAIQAAHDSVAQEGQCMMFR	Cys 152	6	5.87	686.3021	3	2056.891819
			NEAIQAAHDSVAQEGQCDMFR	Cys 152	4	4.54	690.9748	3	2070.910007
Isoform Mt-VDAC1 of Voltage-dependent anion-selective channel protein 1 [VDAC1]	88.69	17	YQVDPDACMMFFSAK	Cys 245	11	1.67	737.3118	2	1473.610765
T-complex protein 1 subunit beta [TCPB]	69.35	24	HGINCMMFFINR	Cys 289	2	1.03	602.2833	2	1203.559374
T-complex protein 1 subunit alpha [TCPA]	54.5	19	LACMMFKEAVR	Cys 125	3	1.16	510.2606	2	1019.514086
Elongation factor 2 [EF2]	52.91	37	STLTDSLVCMMFK	Cys 41	1	1.95	508.7895	2	1196.571825
Heat shock protein HSP 90-beta [HS90B]	76.93	32	VFIMOXDSCMMFDELIPEYLNFIR	Cys 366	2	1.16	1232.073	2	2463.138719
			VFIMOXDSCDMFDELIPEYLNFIR	Cys 366	3	1.98	826.3831	3	2477.164843
Heat shock protein HSP 90-alpha [HS90A]	65.48	27	VFIMOXDNCDMFEELIPEYLNFIR	Cys 375	5	2.77	840.0639	3	2518.177158
Glyceraldehyde-3-phosphate dehydrogenase [G3P]	72.07	8	AAICMMFSGK	Cys 22	3	1.37	390.1814	2	779.3555163
IgE-binding protein [IGEB]	46.68	18	QQCMMFAER	Cys 187	1	1.4	432.678	2	864.3488635
			LQGPPYAESPPCMMFVVR	Cys 181	2	2.09	871.9227	2	1742.838182
Transgelin-2 [TAGL2]	71.86	14	NMOXACMMFVQR	Cys 124	2	2.24	484.2015	2	967.3957995
Collapsin Response Mediator Protein 2 [DPYL2]	61.89	17	GLYDGPVCMMFEVSVTPK	Cys 504	13	3.45	846.4002	2	1693.79326
Asparagine synthetase (glutamine-hydrolyzing) [ASNS]	64.88	20	ETFEDCDMFNLLPK	Cys 455	3	2.36	726.838	2	1452.668383
Tubulin alpha-1A chain [TBA1A]	74.94	12	TIQFVDWCDMFPTGFK	Cys 347	2	2.64	843.3975	2	1685.787767
Tubulin beta-5 chain [TBB5]	75.23	5	TAVCDMFDIPPR	Cys 354	1	-	558.6467	2	1115.5401