Fig. 3.

Profiling of multiplexed Tyr phosphorylation and Lys acetylation for 102 TCGA ovarian tumor samples. A, Preparation of total lysates for 102 TCGA ovarian tumor samples. B, Representative cropped image of HuProt array for multiplexed Tyr phosphorylation and Lys acetylation assay. For example, the Tyr residues of Cofilin-1(CFL1), Putative MAPK-activating protein PM28 (IST1), MAP kinase-interacting serine/threonine-protein kinase 2(MKNK2), and Fibroblast growth factor receptor 1 (FGFR) were heavily phosphorylated by both TCGA samples #1H1 and #1H4. Although, Leucine-rich repeat-containing protein 46 (LRRC46) and LOC285401 were both acetylated by the same two TCGA tumor samples as detected with a pan anti-Kac antibody. C, Number of significantly Tyr-phosphorylated and Lys-acetylated positive hits for each of the 102 TCGA ovarian tumor samples.