Fig. 5.

The focused peptide library was assayed against a panel of kinases in a limited test of FLT3 specificity. A–F, Phosphorylation level for each peptide (rows) at two time points (2 and 60 min, columns) is shown separately for each kinase, with color scales normalized to minimum/maximum RFU values from ELISA detection for that kinase. FAStide candidate data shown in rows 1–8, reference peptide data show in row 9 as a positive control for kinase activity. G, PMS scores for each FAStide candidate and two reference peptides (rows) for 13 kinases (columns) previously built into the KINATEST-ID Screener tool from literature curated substrate input lists. Scores are indicated by color scale from low (white) to high (orange). H, “Off-target” activity prediction from Screener tool, indicating the number of kinases predicted to tolerate a given sequence as a substrate.