Fig. 4.

Comparison of dynamic phosphorylation sites following SH-BC-893 and LB-100 treatments. A, Venn diagram showing that 292 phosphosites are common to both treatments whereas 253 and 98 phosphosites are uniquely regulated by LB-100 and SH-BC-893, respectively. Of the 292 common sites, 116 sites showed opposite (Bidirectional) tends and 176 sites are regulated in the same direction (Monodirectional). B, Phosphorylation motifs extracted from motif-X. Monodirectional phosphosites display PxSP and SP motifs. Bidirectional sites that show rapid changes in phosphorylation are present on Serine-rich peptides. C, Gene Ontology enrichment of proteins corresponding to the 214 bidirectional and unidirectional (for SH-BC-893) phosphosites.