FIG 3.

Ca²⁺ content of forespore and mother cell in sporulating cells. Strains were grown with shaking in 2×SG medium at 37°C and OD₆₀₀ was measured. Samples were removed at T₆ or T₈ and treated with lysozyme and several rounds of centrifugation to separate forespore and mother cell compartments. The samples were extracted with HCl and Ca²⁺ was quantified using atomic emission spectroscopy. Values give the averages from three assays, and error bars indicate standard deviations.