TABLE 6.
Production of heat-resistant spores by B. subtilis ion transporter mutants with different Ca2+ concentrationsa
| Concn (mM) of added CaCl2 | Strain | Total CFU/ml | Heat-resistant CFU/ml |
|---|---|---|---|
| 1 | PS832 | 3.9 × 107 | 4.5 × 107 |
| DPVB693 | 5.8 × 107 | 4.0 × 107 | |
| DPVB706 | 4.2 × 107 | 5.0 × 107 | |
| DPVB722 | 2.9 × 107 | 6.7 × 107 | |
| 0.2 | PS832 | 3.6 × 107 | 3.1 × 107 |
| DPVB693 | 2.8 × 108 | 3.5 × 107 | |
| DPVB706 | 2.7 × 108 | 3.2 × 107 | |
| DPVB722 | 6.3 × 107 | 3.2 × 107 | |
| 0.04 | PS832 | 3.9 × 107 | 4.2 × 106 |
| DPVB693 | 1.4 × 108 | 5.7 × 106 | |
| DPVB706 | 6.8 × 107 | 4.5 × 106 | |
| DPVB722 | 1.4 × 108 | 3.5 × 106 | |
| 0 | PS832 | 3.4 × 107 | 2.5 × 106 |
| DPVB693 | 3.9 × 107 | 9.3 × 105 | |
| DPVB706 | 2.9 × 107 | 2.6 × 106 | |
| DPVB722 | 8.1 × 107 | 3.7 × 105 |
a
Values are averages of two independent experiments.