TABLE 2.
Primers designed and used in this study
| Primer by use | Primer sequence (5′–3′) | Target gene |
|---|---|---|
| Deletion | ||
| AF | ATGAGCTATACCCTGCCATCCCTGCCGTATGCTTACGATGCCCTGGAACCGCACTTCGATAAGCAGACCATTGTGTAGGCTGGAGCTGCT | sodA |
| AR | TTATTTTTTCGCCGCAAAACGTGCCGCTGCTTCGTCCCAGTTCACCACGTTCCAGAACTCTTTAATGTAGATGGGAATTAGCCATGGTCC | |
| Complementation | ||
| RAF | CTCAAGCTTCCATCGTAATCGCGTTACa | sodA |
| RAR | CTCTCTAGATTATTTTTTCGCCGCAAAACGb | |
| RT-qPCR | ||
| katE RT-F | TTGTGGGAAGCCATTGAA | katE |
| katE RT-R | GCGATTGAGCACCATTTT | katE |
| perR RT-F | AGATGACGCCACCCAATA | perR |
| perR RT-R | GGGCATACCAGTTTACCG | perR |
| osmC RT-F | CGGGATTCACGCCAACAT | osmC |
| osmC RT-R | CGGCACCGCAACTTCACT | osmC |
| sodB RT-F | TATCACTACGGCAAGCACC | sodB |
| sodB RT-R | CAGGCAGTTCCAGTAGAAAGTA | sodB |
| sodC RT-F | ACATGAAGGGCCAGAAGG | sodC |
| sodC RT-R | ATATTATCGCCGCCAACG | sodC |
a
HindIII restriction site is in bold.
b
XbaI restriction site is in bold.