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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Cell Stem Cell. 2018 Nov 29;24(2):227–239.e8. doi: 10.1016/j.stem.2018.11.007

Figure 7. Dynamics of replacement of donor HSPCs within the graft by recipient cells.

Figure 7.

(A) Representative flow cytometric gating strategy using jejunum LPLs taken on POD1544 from Pt2 small bowel resection. Lin−:CD3−CD5−CD14−CD19−. The percentage of CD34+ cells among DAPI−CD45+/dimLin− LPLs (B) and the percentage of each cell type of donor vs recipient origin (C). DAPI−Lin−CD45+/dimCD34+ cells, HSC, MPP, LMPP, CLP, MP and CD56+ progenitors in gut LPLs were collected from different patients during stoma revision/closure or small bowel removal at multiple time points post-Tx (POD28–1606). (D) Composition by percentage of HSC, MPP, LMPP, CLP, and MP among donor or recipient DAPI−Lin−CD45+/dimCD34+ LPLs in different patients at multiple time points post-Tx (refer to Figure 7A). Percentage of HSCs (E) and MPs (F) among donor or recipient Lin−CD45+/dimCD34+ LPLs isolated from patients’ intestinal mucosa after 100 days post-Tx shown in box-and-whisker plots (the line inside the box indicates the median). A two-tailed unpaired student t test was used to compare the donor and recipient compartments. (G) Percentages of donor T cells in peripheral blood (left panel) and donor HSCs in gut (right panel) up to 2000 days post-Tx. (H) Percentage of donor HSCs in the gut and the percentage of donor T cells in the blood at a similar timepoint (POD>100) for individual specimens from patients (circles for MVTx, squares for LITx, and triangles for iITx) who underwent stoma revision/closure or small bowel removal at various time points post-Tx. Chi-square test was performed with a cut-off for donor chimerism of 0.2% in peripheral blood (for T cells) or intestinal allograft (for HSCs). See also Figure S1 and Table S1, S2.