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. 2019 Feb 27;12:1641–1653. doi: 10.2147/OTT.S184555

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© 2019 Sun et al. This work is published and licensed by Dove Medical Press Limited

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TCTP silencing in A549 cells using lentivirus-based short-hairpin RNA (Lv-shTPT1).

Notes: (A) Representative images of GFP expression in A549 cell lines in both Lv-shCon group and Lv-shTCTP group. (B) The expression of TCTP, E-cadherin, α-SMA, ZEB1, and p53 in the parental and TPT1-silenced A549 cell lines was examined by Western blot. (C) Bar graph of the expression levels of the proteins in (B). (D) The expression of TCTP, E-cadherin, α-SMA, ZEB1, and p53 in the parental and TCTP-silenced A549 cell lines was examined by confocal laser microscopy. (E) Bar graph of the expression levels of the proteins in (D). *P<0.01.