Table 1. Table showing the compounds used to investigate mitochondrial complex activity.

Compound(s)	10× port solution concentration	Final in well concentration	Complex investigated
Pyruvate/malate	50 mM/25 mM	5 mM/2.5 mM	Complex I mediated respiration
Succinate	100 mM	10 mM	Complex II mediated respiration
Tetamethylphenylendiamine (TMPD)/Ascorbate	5 mM/20 mM	0.5 mM/2 mM	Complex IV activity
Palmitoyl-l-carnitine	0.5 mM	50 µM	Respiratory activity supported by fatty acid oxidation
Glutamine/malate	40 mM/5 mM	4 mM/0.5 mM	Respiratory activity supported by glutaminolysis
ADP	10 mM	1 mM	State 3 respiratory activity
Saponin	250 µg/ml	25 µg/ml	Cell permeabilisation
FCCP	10 µM	1 µM	Maximal respiratory activity
Oligomycin	10 µM	1 µM	State 4 respiratory activity
Rotenone	10 µM	1 µM	Metabolic inhibitor
Potassium Azide	200 mM	20 mM	Metabolic inhibitor