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. 2019 Feb 15;146(4):dev168146. doi: 10.1242/dev.168146

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Genes involved in immunoresponses, cell cycle and early tissue development manifest increased expression in postnatal Cnot3^−/− livers. (A-D) qPCR analysis of mRNAs in livers from E16.5, 1-week-old (1 w) and 4 w control and Cnot3LKO mice. Gapdh mRNA levels were used for normalization. Expression levels of genes in control mice (1 w in A, 4 w in B,C; 8 w in D) are set to 1 (n=5). Data are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001. (E,G) Immunoblot of tissue lysates from control (Ctrl) and Cnot3LKO (KO) mice. (F) Immunohistochemistry for Afp and Igf2 using 4 w control and Cnot3^−/− livers. Scale bars: 50 μm.