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. 2019 Feb 15;8(2):bio041244. doi: 10.1242/bio.041244

Fig. 2.

Fig. 2.

Cross experiment demonstrates the relationship between mtDNA and Gcn5. To obtain diploid competent for respiration it is required that the mtDNA does not derive from gcn5Δ strain. (A,B) Schematic procedure of the two crosses to reintroduce GCN5 gene in the deleted strain. Nucleus with wild-type chromosomal DNA is grey whereas in the presence of GCN5 deletion it is black. MtDNA is shown as small beads; GLY+ or − indicate the capability to grow in glycerol containing medium. (C,D) Serial dilutions of parental strains and diploids of the crosses described in A and B, respectively. (E) Serial dilutions of WT, gcn5Δ and spore W303.2b (obtained from the cross described in A) in which the wild-type phenotype is completely restored. All strains are spotted on YP 2% glucose or in 3% glycerol containing media and incubated at 28 or 37°C.