Fig. 7.

Hepatic IRA expression alters mRNA levels of key genes involved in hepatic lipid metabolism. mRNA levels of Cd36, Fasn, Pgc1a, Scd1, Acaca and Dgat2 by quantitative RT-PCR in STD (n=5), HFD (n=9), HFD-GFP (n=6), HFD-IRA (n=8) and HFD-IRB (n=7) mice. 36b4 was used as a control. Results are expressed as mean±s.e.m. Statistical significance was assessed by two-tailed unpaired Student's t-test [versus STD mice (*P<0.05 and **P<0.01), versus HFD mice (^$P<0.05 and ^$$P<0.01), versus HFD-GFP mice (^±P<0.05, ^±±P<0.01 and ^±±±P<0.001) and versus HFD-IRB mice (^†P<0.05 and ^††P<0.01)].