Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 12;12(2):dmm036863. doi: 10.1242/dmm.036863

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 3. — Monitoring of cfDNA levels using Gapdh analysis by qPCR. (A) Gapdh levels as measured by qPCR in cfDNA in comparison to tumour burden in Kras^+/LSL-G12D mice over a time course following infection with Ad5-CMV-Cre (red) or Ad5-CMV-βgal (blue). Total tumour burden was quantitated from micro-CT imaging. Gapdh levels were measured by qPCR of circulating DNA derived from the plasma of mice at each time point. Mean values are indicated by diamonds/lines; values for individual mice are indicated by circles (n=3-12 at each time point). *P<0.05 for two-tailed unpaired Student's t-test comparisons between mean values at a given time point and values at time=0. The correlation coefficients between Gapdh levels and tumour burden are indicated (linear regression analysis; P-value, goodness of fit). (B) Gapdh levels in cfDNA as measured by qPCR in comparison to tumour burden in Kras^+/LSL-G12D mice over a time course following infection with Ad5-mSPC-Cre. Mean values are indicated by diamonds/lines; values for individual mice are indicated by circles (n=4-9 at each time point). *P<0.05 for two-tailed unpaired Student's t-test comparisons between mean values at a given time point and values at time=0. The correlation coefficients between Gapdh levels and tumour burden are indicated (linear regression analysis; P-value, goodness of fit).