Fig. 2.
Effects of manipulation of food supplementation on hepatocarcinogensis and muscle wasting. 4-month-old male adult krasG12V and WT zebrafish were treated with dox for 4 weeks and fed with different levels of artemia. A total of 5 mg artemia cysts/fish/day for normal feeding was given, defined as 100%. There were two underfeeding groups with 25% and 50% the amount of food of normal feeding, and two overfeeding groups with 200% and 300% the amount of normal feeding. In each feeding group, 20 fish were used to initiate the experiments. (A) Gross appearance and liver morphology based on liver-specific GFP expression in krasG12V fish. (B) Survival curves. (C) Body weight excluding internal viscera. (D) H&E staining of liver sections of krasG12V fish (left) and quantification (right). (E) IF staining of PCNA (red), Hnf4a (green) and DAPI (blue) in liver sections. Quantification of percentage of proliferating hepatocytes (right). (F) H&E staining of muscle sections of krasG12V fish. Quantification of MFCSA of krasG12V fish (right). *P<0.05. Scale bars: 2.5 mm in A; 10 μm in D-F.