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. 2019 Feb 25;10:287. doi: 10.3389/fimmu.2019.00287

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Copyright © 2019 Guo, Liu, Yin, Kan, Gong, Li, Cao, Wang, Xu, Ma, Fu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of licochalcone A on the protein levels of ZO-1, Occludin, and claudin3 in mMECs. mMECs were serum-starved for 3 h before treatment with Licochalcone A (1.8, 2.4 μg/mL) for 24 h (A). The protein levels of ZO-1 (B), claudin3 (C), and Occludin (D) were determined by western blot, and the relative protein levels were quantified by scanning densitometry and normalized to β-tubulin. Values are presented as means ± SD (n = 3) (**p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. Control group).